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KMID : 0364020100430050499
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Korean Journal of Thoracic and Cardiovascular Surgery
2010 Volume.43 No. 5 p.499 ~ p.505
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Expression of CD40, CD86, and HLA-DR in CD1c＋ Myeloid Dendritic CellsIsolated from Peripheral Blood in Primary Adenocarcinoma of Lung
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Kang Moon-Chul
Kang Chang-Hyun
Kim Young-Tae
Kim Joo-Hyun
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Abstract
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Background:There have been several reports using animal experiments that CD1-restricted T-cells have a key role in tumor immunity. To address this issue, we studied the expression of markers for CD1c＋ myeloid dendritic cells (DCs) isolated from peripheral blood in the clinical setting.
Methods:A total of 24 patients with radiologically suspected or histologically confirmed lung cancer who underwent pulmonary resection were enrolled in this study. The patients were divided according to histology findings into three groups: primary adenocarcinoma of lung (PACL), primary squamous cell carcinoma of lung (PSqCL) and benign lung disease (BLD). We obtained 20 mL of peripheral venous blood from patients using heparin-coated syringes. Using flow-cytometry after labeling with monoclonal antibodies, data acquisition and analysis were done.
Results:The ratio of CD1c＋CD19? dendritic cells to CD1c＋ dendritic cells were not significantly different between the three groups. CD40 (p=0.171), CD86 (p=0.037) and HLA-DR (p=0.036) were less expressed in the PACL than the BLD group. Expression of CD40 (p=0.319), CD86 (p=0.036) and HLA-DR (p=0.085) were less expressed in the PACL than the PSqCL group, but the differences were only significant for CD86. Expression of co-stimulatory markers was not different between the PSqCL and BLD groups. Expression of markers for activated DCs were dramatically lower in the PACL group than in groups with other histology (CD40 (p=0.005), CD86 (p=0.013) HLA-DR (p=0.004).
Conclusions:These results suggest the possibility that CD1c＋ myeloid DCs participate in control of the tumor immunity system and that low expression of markers results in lack of an immune response triggered by dendritic cells in adenocarcinoma of the lung.
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KEYWORD
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Immunology, Adenocarcinoma, Lung neoplasm, Dendritic cells, Antigens
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